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Korean J Med > Volume 86(4); 2014 > Article
The Korean Journal of Medicine 2014;86(4): 444-452.
일측성 요로폐쇄 흰쥐 신장에서 암모니아 운반체의 발현
김승중1, 김혜영1, 최재현1, 이동화1, 김경민1, 김선문1, 권순길1, 신경섭2, 김경숙3
1충북대학교 의과대학 내과학교실, 의학연구소
2충북대학교 의과대학 진단검사의학과교실
3충북대학교 약학대학 임상약학실
Renal Expression of an Ammonia Transporter in Rats with a Unilateral Ureteral Obstruction
Seung Jung Kim1, Hye-Young Kim1, Jae Hyun Choi1, Dong Hwa Lee1, Kyung Min Kim1, Sun Moon Kim1, Soon Kil Kwon1, Kyung Sub Shin2, Kyung Sook Kim3
1Departments of Internal Medicine, Medical Research Institute, Chungbuk National University College of Medicine, Cheongju, Korea
2Departments of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Korea
3Clinical Pharmacy, Chungbuk National University College of Pharmacy, Cheongju, Korea
Corresponding Author: Hye-Young Kim ,Tel: +82-43-269-6017, Fax: +82-43-273-3252, Email: hyekim@chungbuk.ac.kr
Received: June 11, 2013;   Revised: July 8, 2013;   Accepted: August 19, 2013.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
ABSTRACT
Background/Aims:
Urinary tract obstruction induces a form of renal tubular acidosis with a urinary acidification defect caused by decreasing net acid excretion, which is predominantly due to a decrease in urinary ammonia excretion. The present study examined whether this decrease is associated with changes in the renal expression of an ammonia transporter family member, Rh C glycoprotein (Rhcg), in rats with a unilateral ureteral obstruction.
Methods:
Male Sprague-Dawley rats underwent a 24-h unilateral ureteral obstruction. Rhcg expression was then evaluated by immunoblotting and immunohistochemistry. Cell height, total cellular expression, expression in the apical 25% of the cell, and % of total expression in the apical region were quantified by immunohistochemistry with quantitative morphometric analysis.
Results:
After 24 h of unilateral ureteral obstruction, the serum bicarbonate level and total urinary ammonia excretion were decreased. Both light microscopy and immunohistochemistry with quantitative morphometric analysis demonstrated that the total intensity of Rhcg expression was decreased in the obstructed kidneys, whereas Rhcg expression did not change in the cortical collecting duct (CCD) and outer medullary collecting duct (OMCD) of nonobstructed kidneys in rats with a 24-h unilateral ureteral obstruction.
Conclusions:
The rats with a unilateral ureteral obstruction showed decreased urinary ammonia excretion associated with decreased Rhcg expression in the CCD and OMCD. These changes suggest that the ammonia transporter Rhcg mediates a urinary acidification defect associated with unilateral ureteral obstruction. (Korean J Med 2014;86:444-452)
Keywords: Ureteral obstruction; Acidosis; Ammonia; Kidney tubules; Collecting
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