| Case Reports : Identification of Gemella species by 16S ribosomal RNA gene sequencing from two patients with infective endocarditis |
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Kyung Mok Sohn, Kwan Soo Ko, Jeong Kim, Ji Young Rhee, Won Sup Oh, Kyong Ran Peck, Jae Hoon Song |
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한림의대 한강성심병원 내과 |
| 증례 : 감염성 심내막염 환자에서 16S rRNA 유전자 염기서열 분석을 통한 Gemella 동정 2예 |
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손경목, Kwan Soo Ko, Jeong Kim, Ji Young Rhee, Won Sup Oh, Kyong Ran Peck, Jae Hoon Song |
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| Abstract |
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Gemella species are opportunistic pathogens, which cause endocarditis, meningitis, musculoskeletal infections, or pulmonary infections. It is difficult to identify all strains of these species by a conventional culture system. Because 16S rRNA gene has the interspecific polymorphisms to differentiate the bacterial species, 16S rRNA gene sequence analysis could be used to identify these pathogens. To determine the molecular types of two unidentified Gram-positive cocci from two patients with infective endocarditis, PCR amplification and DNA sequencing of the 16S rRNA gene were performed. These sequences of the PCR product were compared with known 16S rRNA gene sequences using GenBank BLAST search. The 16S rRNA gene sequences of two isolates showed >
99% nucleotide similarities with those of Gemella morbillorum (GenBank accession number L14327). Phylogenetic analysis also indicated the close relatedness between these isolates and G. morbillorum. (Korean J Med 70:591-596, 2006)
Key Words : Gemella, 16S rRNA, Endocarditis |
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