-
Review
-
Korean J Med. 2007;73(2):541-550.
- Characterization of buckwheat 19 kD allergen and its application for diagnosing clinical reactivity
-
박중원
-
- Characterization of buckwheat 19 kD allergen and its application for diagnosing clinical reactivity
-
Jung-Won Park
-
한림대학교 의과대학 내과학교실 강동성심병원 심혈관센터
-
- Abstract
- Background : The 19 kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined.
Methods : cDNA of 19 kD BW allergen was cloned and expressed in E. coli. Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant protein (r19 kD) was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW allergic and 19 BW asymptomatic sensitized subjects using ROC analysis.
Results : The 19 kD BW allergen, which is composed of 135 amino acids, has weak homology to the vicilin like allergens of cashew (Ana o 1), English walnut (Jug r 2) and 7 S globulin from Sesamum indicum. r19 kD can inhibit sIgE binding to native 19 kD BW allergen. The maximum % inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of BW allergy patients had sIgE bound to r19 kD, compared to only 1 of the 19 BW asymptomatic sensitized subjects. The areas under the ROC curves for the skin prick tests [0.925 (95% CI: 0.839～1.012), p<0.001) as well as r19 kD sIgE ELISAs [0.860 (0.725～0.995), p <0.001] were higher than that of BW sIgE CAP test results [0.803 (0.661～0.945), p=0.002].
Conclusions : The 19 kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19 kD sIgE ELISA test was more discriminative than the CAP sIgE measurement using whole BW extract.
Keywords :Buckwheat allergen, major allergen, 19 kD allergen, clinical reactivity
