| Acute gut injury and bacterial translocation in a rat model with combined oral administration of methotrexate and diclofenac |
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Jeong Wook Kim |
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1Department of Cardiology, Catholic University of Daegu College of Medicine, Daegu, Korea |
| 원저 : Diclofenac과 methotrexate 병합 경구 투여한 백서에서의 급성 장관손상과 장내세균전위 |
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김정욱 |
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| Abstract |
Background : NSAIDs and methotrexate induce gut damage and bacterial translocation (BT). However, there is no study examining the combined effects of methotrexate and NSAID on gut damage and BT. We examined the combined effects of methotrexate and NSAID-induced enteropathy and bacterial translocation in an experimental animal model.
Methods
: Rats received either no drug, NSAID alone (diclofenac 80 mg/kg and 120 mg/kg per os), methorexate alone (20 mg/kg per os) or NSAID with methotrexate. Gut barrier dysfunction, the degree of intestinal adhesion, stool pellet number, bacterial number of total aerobes and Gram negatives in the distal ileal and cecal contents and the number of Gram negatives in the mesenteric lymph nodes, liver, spleen, kidney and heart were measured.
Results
: Administration of diclofenac or methotrexate alone caused an increase in gut barrier dysfunction and intestinal adhesion and a decrease in stool pellet number. Administration of diclonfenac alone induced enteric bacterial overgrowth and increased BT to the mesenteric lymph nodes, liver, spleen, kidney and heart. Administration of methotrexate alone induced enteric bacterial undergrowth and BT to the mesenteric lymph nodes, liver, spleen but not to the kidney and heart. The supplements with methotrexate increased the NSAID-induced gut barrier dysfunction and intestinal adhesion, and decreased the stool pellet number. However, the reduced NSAID-induced enteric Gram negative bacterial overgrowth (with a dose of diclofenac of 80 mg/kg) and BT to the liver, spleen, kidney and heart.
Conclusion
: Methotrexate increases NSAID-induced intestinal damage, but reduces NSAID-induced BT to the liver, spleen, kidney and heart in experimental animals.(Korean J Med 73:258-266, 2007)
Key Words : Anti-inflammatory agents, Non-steroidal, Methotrexate, Bacterial translocation, Adverse effects |
| Key Words:
Anti-inflammatory agents, Non-steroidal, Methotrexate, Bacterial translocation, Adverse effects |
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