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Mi Ok Kim, Tae Hyung Kim, Jang Won Sohn, Ho Joo Yoon, Dong Ho Shin, Sung Soo Park |
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서울대학교 의과대학 내과학교실, 간연구소 |
| 원저 : 스테로이드제 투여 후 친수성과 공수성 표면활성물질 단백의 전사 신장도 비교 (Original Articles : The comparative assessment of nuclear run on of hydrophilic and hydrophobic surfactant protein by administration of ste |
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김미옥, 김태형, 손장원, 윤호주, 신동호, 박성수 |
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| Abstract |
Background : Surfactant proteins are important in the regulation of the surfactant secretion,
synthesis and recycling. Glucocorticoids are known to have primary or secondary effects on gene
expression and can alter the rate of gene transcription. The hydrophilic and hydrophobic surfactant
protein have been shown to be upregulated by glucocorticoids in vitro but its regulation in vivo,
however, is not well established. The authors carried out nuclear run on assays to determine
wheather glucocorticoids altered the transcription rate of SP-A, SP-B and SP-C genes.
Methods
: Adult rats were given the 2 mg/kg dose of subcutaneous dexamethasone for 2 days
and sacrified at 2 days. The transcription rate of SP-A, SP-B and SP-C genes were measured by
nuclear run on assays.
Results
: Treatment with 2 mg/kg dexamethasone increased transcription of SP-A gene (1.6-fold)
and SP-C gene (1.3-fold) compared to the control for SP-A and SP-C after 2 days, which were not
statistically significant. The rate of gene transcription for SP-B at 2 days after 2 mg/kg
dexamethasone administration was significantly increased by 5.3-fold compared to the control for
SP-B (p<0.005). The rates of gene transcription for hydrophilic and hydrophobic surfactant proteins,
even in the hydrophobic surfactant proteins SP-B and SP-C were different.
Coclusions : The authors conclude that the difference in dexamethasone sensitivity may indicate
that the three surfactant protein genes contain glucocorticoid response elements with different
affinities for receptor in vivo.(Korean J Med 70:53-60, 2006)
Key Words : Nuclear run on, Dexamethasone, Pulmonary surfactant |
| Key Words:
Nuclear run on, Dexamethasone, Pulmonary surfactant |
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