| Effect of Rosiglitazone on Adipose Tissue Redistribution in a Diabetic Animal Model |
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1Department of Internal Medicine, Hanyang University College of Medicine, Seoul; 2Department of Life Science, Postech Biotech Center, Pohang University of Science and Technology, Pohang, Korea |
| 당뇨병 동물모델에서 Rosiglitazone의 투여가 지방 재분포에 미치는 영향 |
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강준구 |
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| Abstract |
Background : Treatment with Thiazolidinediones(TZDs) improves glucose homeostasis by increasing insulin sensitivity, but leads to weigh gain. Many studies reported that TZDs increase subcutaneous adiposity but have no effect on the visceral fat mass. The mechanism of this phenomenon has not yet been fully elucidated. My hypothesis in adipocytes is that there is a depot
specificity of lipid storage & energy expenditure gene regulation by TZD treatment.
The aim of this study is to define effect of TZDs on transcription of lipogenic, lipid storage and energy expenditure genes in adipose tissue and liver in vivo.
Methods
: Otsuka Long-Evans Tokushima Fatty (OLETF) rats and control LETO rats were fed with chow or chow containing the rosiglitazone for 5 weeks.
Body weight and IPGTT were checked in pre- and post treatment. We used a real-time RT-PCR assay to quantify mRNA level in subcutaneous and visceral fat tissues.
Results
: The body weight was more increased in TZD-treated OLETF rats than in untreated OLETF rats. In IPGTT,
The glucose level was lower in TZD-treated OLETF rats than in untreated OLETF rats. Subcutaneous and epididymal fat mass was increased after TZDs treatment in OLETF rat. In RT-PCR analysis, glycerol and FA substrate related gene expressions were increased in subcutaneous fat tissue. In visceral fat tissue, lipolysis related gene expression was increased, but de novo synthesis of FA was decreased and Beta-oxidation related gene expression was increased.
Conclusion
: Depot-specific effect of TZDs might be due to difference between energy storage and expenditure gene expression.
Key Words : Thiazolidinedione, Fat distribution, OLETF rat |
| Key Words:
Thiazolidinedione, Fat distribution, OLETF rat |
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