In Vitro Effects of Antibiotics against Multidrug-resistant Pseudomonas aeruginosa |
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1Department of Internal Medicine, Hanyang University College of Medicine, Seoul; 2Department of Life Science, Postech Biotech Center, Pohang University of Science and Technology, Pohang, Korea |
다약제 내성 Pseudomonas aeruginosa의 항생제 효과에 관한 연구 |
배 현 주 |
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Abstract |
Objects : The inhibitory effects of combination of β-lactam with ciprofloxacin or amikacin against clinical isolates of multidrug-resistant P. aeruginosa was evaluated.
Methods : Ten isolates with variable level of resistance to ceftazidime, cefepime, piperacillin/ tazobactam, meropenem, ciprofloxacin and amikacin were included in this study.
Efficacy of combined antibiotics was studied using a checkerboard method or in vitro killing assay.
Results : Combination of β-lactam/carbapenem with amikacin showed synergistic effects for all the strains regardless of MIC level of amikacin, but that with ciprofloxacin showed synergistic effect for the isolate with low MIC of ciprofloxacin by checkerboard method. The isolates with high MIC of ciprofloxacin showed indifferent effect on combination of β-lactam/carbapenem and ciprofloxacin. In vitro killing assay showed that carbapenem with ciprofloxacin effected synergistically for the isolates with MIC of 16 μg/mL of ciprofloxacin. Carbapenem with amikacin also showed synergistic effects for the isolates even with MIC of 128 μg/mL of amikacin. However, no synergy was observed for the combination of ceftazidime/piperacillin-tazobactam and amikacin.
Conclusion : Carbapenem with amikacin can be the first choice for the infections caused by pan-drug resistant P. aeruginosa, when the level of resistance was not identified.
Key Words : all-drug resistant P. aeruginosa, synergy, β-lactam, carbapenem, aminoglycoside, fluoroquinolone |
Key Words:
all-drug resistant P. aeruginosa, synergy, β-lactam, carbapenem, aminoglycoside, fluoroquinolone |
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